Details
Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.
Kit features:
1. It specifically detects Chlamydia psittaci and has no cross-reaction with the genomes of other organisms.
2, the sensitivity is significantly higher than that of serological methods, About ten times higher than ordinary PCR method.
3. DNA polymerase adopts hot start method. It can inhibit non-specific amplification and reduce background fluorescence.
4, with positive control sample, can be used Check the effectiveness of the kit.
5, with UDG enzyme and dUTP, can Reduce residual DNA contamination.
6, with ROX reference dye, help Correct for loading errors and tube-to-tube differences.
Chlamydia psittaci, also known as Chlamydia psittaciphila, Chlamydia psittaciphila, is A strictly intracellular parasitic prokaryotic organism, belonging to the family Chlamydiaceae . Chlamydia psittaci is the earliest discovered chlamydia, and its host is very wide, including not only a variety of birds, including parrots, but also There are many mammals; unique subtypes have been formed in many hosts, and some subtypes have been divided into independent species, such as: Chlamydia pecorum, Chlamydia abortus, guinea pig chlamydia, cat chlamydia, etc. Chlamydia psittaci can be transmitted through direct contact, bird droppings, nasal Secretions and aerosols, causing respiratory disease in birds and mammals; can pose a public safety threat, including large-scale outbreaks in poultry production, and in humans because it can remain infectious in the environment for months Occasionally caused by psittacosis . Humans are usually infected from domestic or wild birds. Human-to-human transmission has yet to be confirmed. Humans often have no symptoms or flu-like symptoms after infection. , sometimes progressing to severe pneumonia, endocarditis, or encephalitis. Chlamydia psittaci is divided into many genotypes, including: A–F, M56, and WC. Each subtype has strict host specificity. Subtype A The host is Psittacidae, B host is pigeon, C host is duck and goose, D host is turkey, E host is pigeon, duck and other birds, F host is parrot, WC host is cow, and M56 host is Rodents.
Chlamydia psittaci can be detected using culture and serological methods Detection. Chlamydia psittaci is easy to grow in chicken embryo yolk sac, HeLa cells, and monkey kidney cell cultures. However, because it is time-consuming and laborious, and requires high skills and equipment from the experimenter, the culture method is rarely used. Some serum methods, Such as: complement fixation test, indirect hemagglutination test technology, ELISA, micro-immunofluorescence method, commonly used to detect parrotsThe disadvantage of thermochlamydia is that it may cross-react with other chlamydiae and has low sensitivity, making it not suitable for early infections. The PCR method is an in vitro enzymatic synthesis method of specific DNA fragments. It is more suitable for detecting Chlamydia psittaci. It has high sensitivity, strong specificity, and fast detection speed, which can be completed in only two or three hours. Compared with the ordinary PCR method, the quantitative PCR method can not only accurately quantify, but also be more convenient to operate and less affected by environmental pollution.
This kit designs primers for the 16S-23S rRNA gene interval sequence, which can be specific Sexually identifying Chlamydia psittaci, verified by BLAST, there is no cross-reactivity with other biological genomes. This kit was used to detect 9 different types of chlamydia, 20 types of bacteria and 4 types of viruses that may cause similar symptoms, and no non-specific signals were found; 327 bird samples were tested and 47 positive results were obtained. It can be seen that this kit is suitable for the detection and identification of Chlamydia psittaci.
-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。
试剂盒特点：
1， 特异性检测鹦鹉热衣原体，与其他生物基因组无交叉反应。
2， 灵敏度明显高于血清学方法，大约高于普通PCR法十倍。
3， DNA聚合酶采用热启动方式，可抑制非特异性扩增，降低背景荧光。
4， 带有阳性对照样品，可用于检验试剂盒有效性。
5， 带有UDG酶和dUTP，可降低残留DNA的污染。
6， 带有ROX参比染料，帮助校正加样误差和管间差异。
鹦鹉热衣原体（Chlamydia psittaci），或称鹦鹉热嗜衣原体、鹦鹉热亲衣原体（Chlamydophila psittaci），是一种严格细胞内寄生的原核生物，属于衣原体科（Chlamydiaceae）。鹦鹉热衣原体是最早发现的衣原体，宿主非常广泛，不仅有包括鹦鹉在内的多种鸟类，还有许多哺乳动物；在许多宿主中已形成独特的亚型，部分亚型已被划分为独立物种，如：兽类衣原体（Chlamydia pecorum）、流产衣原体（Chlamydia abortus）、豚鼠衣原体（Chlamydia caviae）、猫衣原体（Chlamydia felis）等。鹦鹉热衣原体的传播可通过直接接触、鸟粪、鼻分泌物和气溶胶，引起鸟类和哺乳动物的呼吸道疾病；由于可在环境中保持感染力达数月之久，因此可以造成公共安全威胁，包括在家禽养殖中的大规模爆发，以及在人类中偶尔引发的鹦鹉病（或称鸟疫、鹦鹉热）。人类一般是从家养或野生鸟类获得感染，人与人之间的传播尚待证实，人类感染后常没有症状或出现类似流感的症状，有时会发展为严重肺炎、心内膜炎或脑炎。鹦鹉热衣原体分为很多基因型，包括：A–F, M56和WC，每种亚型都具有严格的宿主特异性，A亚型宿主为鹦鹉科鸟类，B宿主为鸽，C宿主为鸭和鹅，D宿主为火鸡，E宿主为鸽、鸭及其他鸟类，F宿主为鹦鹉，WC宿主为牛，M56宿主则为啮齿类动物。
鹦鹉热衣原体可使用培养法和血清学方法进行检测。鹦鹉热衣原体在鸡胚卵黄囊及HeLa细胞、猴肾细胞培养中易于生长,但由于耗时费力，对实验者技能和设备要求也比较高，因此培养法很少使用。一些血清方法，如：补体结合试验、间接血凝试验技术、ELISA、微量免疫荧光法，常用于检测鹦鹉热衣原体，其缺点在于可能与其他衣原体产生交叉反应，且灵敏度较低，不适用于早期感染。PCR法是一种体外酶促合成特异性DNA 片段的方法,更适合检测鹦鹉热衣原体，其灵敏度高，且特异性强，检测速度快，只需两三个小时即可完成。定量PCR法与普通PCR法相比，不仅可以精确定量，而且操作更为方便，更少受环境污染的影响。
本试剂盒针对16S-23S rRNA基因区间序列设计引物，可以特异性识别鹦鹉热衣原体，经BLAST验证，与其他生物基因组没有交叉反应。使用本试剂盒检测了9种不同的衣原体，和可能引起类似症状的20种细菌和4种病毒，未发现非特异性信号；对327个鸟类样本进行检测，获得47个阳性。可见本试剂盒适用于鹦鹉热衣原体的检测和鉴定。
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