Store at -20°C in the dark and avoid repeated freezing and thawing when transported at 2-8°C. Without repeated freezing and thawing, the shelf life is one year.

 

 Ureaplasma classification Belongs to Mycoplasma, one of the smallest free-living organisms. It has no cell wall and limited biosynthetic capacity. It needs to absorb cholesterol from the environment and hydrolyze urea to produce ATP for metabolism. So far, there are 14 known serotypes of Ureaplasma that parasitize humans, belonging to two species: Ureaplasma parvum . The genome size of Ureaplasma parvum ranges from 0.75–0.78 Mbp, and that of Ureaplasma urealyticum ranges from 0.84–0.95 Mbp. There is currently no consistent conclusion on the impact of different serotypes on human diseases. It is generally believed that Ureaplasma is a commensal bacterium in the genitourinary tract of sexually active adults. The positive rate in women is about 40-80%. It is sometimes associated with some diseases, such as: urethritis, postpartum endometritis, chorionic villi Amnionitis, spontaneous abortion, premature birth, low birth weight, pneumonia, bacteremia, meningitis and chronic lung disease of premature infants, etc. It is unclear under what circumstances Ureaplasma causes disease, and people with intact humoral immune systems are generally unaffected.

The in vitro culture of Ureaplasma is very difficult, time-consuming and has low sensitivity. The typing of Ureaplasma relies on monoclonal or polyclonal antibodies against whole cells or purified antigens. Experimental methods include: growth inhibition experiment, metabolic inhibition experiment, immunofluorescence, immunoperoxidase, ELISA, western blot, etc. These methods are time-consuming and labor-intensive, the results are often difficult to reproduce, there are many cross-reactions, and it is often difficult to distinguish between samples containing multiple serotypes. The PCR method used to detect Ureaplasma has higher sensitivity and better specificity, is not affected by contamination from other microorganisms, and has a short detection time, and the results can be obtained in just a few hours.

This kit selects tinyThe Ureaplasma 16S rRNA gene is used as a target and can specifically identify Ureaplasma parvum. It has been verified by BLAST that there is no cross-reaction with Ureaplasma urealyticum and other biological genomes. Detection of 6 other mycoplasma species showed no specific reaction. Detects 14 Ureaplasma serotypes and can correctly distinguish whether they belong to Ureaplasma parvum. Colonies isolated from 78 clinical samples were tested, and 62 positives were obtained, and 1 was mixed positive with Ureaplasma urealyticum; 185 swabs were tested, and 151 positives were obtained, and 14 were mixed positive with Ureaplasma urealyticum. It can be seen that this kit can be used for the detection and identification of Ureaplasma parvum.

This product is for scientific research only.

 

 

 

-20℃避光保存，2-8℃运输避免反复冻融。没有反复冻融的情况下，保质期一年。

 

 

脲原体分类上属于支原体，是最小的可自由生活生物之一。它没有细胞壁，生物合成能力有限，需从环境吸收胆固醇，水解尿素产生ATP以供代谢。迄今为止，寄生于人类的脲原体已知有14个血清型，分属于两个物种：微小脲原体（Ureaplasma parvum，也有译为细小脲原体）（含4个血清型：UPA1, UPA3,UPA6, UPA14）和解脲脲原体（Ureaplasma urealyticum，也有称为溶脲脲原体）（含10个血清型：UUR2, UUR4, UUR5, UUR7, UUR8, UUR9, UUR10, UUR11, UUR12, 和UUR13）。微小脲原体的基因组大小在0.75–0.78 Mbp之间，解脲脲原体的基因组大小在0.84–0.95 Mbp。不同血清型对人体疾病的影响目前尚没有一致结论。一般认为脲原体是性活跃成年人的泌尿生殖道共生菌，在女性中阳性率约在40-80%，有时会与一些疾病相关联，如：尿道炎、产后子宫内膜炎、绒毛膜羊膜炎、自然流产、早产、低出生体重、肺炎、菌血症、脑脊膜炎和早产儿慢性肺病等。目前尚不清楚脲原体会在什么情况下引起疾病，体液免疫系统健全的人一般不会受其影响。

脲原体的体外培养非常困难，耗时且灵敏度较低。对脲原体的分型是依靠针对全细胞或纯化抗原的单克隆或多克隆抗体，实验方法包括：生长抑制实验、代谢抑制实验、免疫荧光、免疫过氧化物酶、ELISA、免疫印迹等，这些方法都比较耗时费力，结果常常难以重复，有很多交叉反应，在含有多个血清型的样本上常难以区分。而PCR法用于检测脲原体，则有更高的灵敏度和更好的特异性，不受其他微生物污染的影响，且检测时间短，仅需几个小时即可获得结果。

本试剂盒选择微小脲原体16S rRNA基因作为靶点，可以特异性识别微小脲原体，经BLAST验证，与解脲脲原体及其他生物基因组没有交叉反应。检测6种其他支原体，均无特异性反应。检测14种脲原体血清型，可正确区分是否属于微小脲原体。检测78个临床样本分离出来的菌落，得到62个阳性，以及1个与解脲脲原体混合阳性；检测185个拭子，得到151个阳性，以及14个与解脲脲原体混合阳性。可见本试剂盒可用于微小脲原体的检测和鉴定。

本产品仅供科学研究使用。

 

 

 

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