Store at -20°C in the dark and avoid repeated freezing and thawing when transported at 2-8°C.

Without repeated freezing and thawing, the shelf life is one year.

 

Mycoplasma pneumoniae is one of the smallest self-replicating organisms and is the human pathogen causing mycoplasma pneumonia, an atypical bacterial pneumonia associated with cold agglutinin disease. Mycoplasma pneumoniae cells are elongated in shape, about 1-2 μm long and 0.1-0.2 μm wide, and cannot be detected under a light microscope; the length of their colonies is usually less than 100 μm and requires a stereomicroscope to observe. Mycoplasma pneumoniae parasitizes the human respiratory epithelium, adheres to respiratory epithelial cells by attaching organelles, and then fuses with host cells and enters the cells, evading detection by the host immune system, and adjusting the composition of the cell membrane to mimic the host cell membrane. Mycoplasma pneumoniae therefore tends to produce chronic or latent infections, and because its cell membrane composition is similar to human cells, it may lead to autoimmune reactions in several organs and tissues .

In vitro culture methods are rarely used to detect Mycoplasma pneumoniae. Possible detection methods include : Western blotting, immunofluorescence staining, hemoadsorption test, tetrazolium reduction, metabolic inhibition test, serology test and polymerase chain reaction, etc. Due to its low cost and relatively short test time, EIA serological analysis is the most commonly used M. pneumoniae detection method for diagnosis. Its disadvantage is that it requires the use of biopsied tissue, and this sampling method may increase the severity of the infection. PCR is the fastest and most effective method to determine the presence of Mycoplasma pneumoniae. It has higher sensitivity, strong detection specificity, and short detection time, with results available in just a few hours.

The Mycoplasma pneumoniae PCR detection kit selected a sequence of the Mycoplasma pneumoniae cytoadherin protein gene for PCR identification, and the primers were verified by BLAST as specific targets. Mycoplasma pneumoniae has no cross-reactivity with the genomes of other organisms. 572 samples were tested using this kit, and 83 positive results were obtained, which were verified by sequencing. It can be seen that this kit is species-specific and can be used for the identification and detection of Mycoplasma pneumoniae.

This kit is for scientific research use only.

 

-20℃避光保存，2-8℃运输避免反复冻融。没有反复冻融的情况下，保质期一年。

 

 

肺炎支原体（Mycoplasma Pneumoniae）属于最小的自我复制型生物之一，是导致支原体肺炎（一种与冷凝集素病有关的非典型细菌性肺炎）的人类病原体。肺炎支原体细胞呈细长形状，长约1-2μm，宽约0.1-0.2μm，光学显微镜下无法检测；其菌落的长度通常小于100μm，需要立体显微镜来观察。肺炎支原体寄生于人类的呼吸道上皮，通过附着细胞器粘附于呼吸上皮细胞，然后与宿主细胞融合并进入细胞内，逃避宿主免疫系统的检测，并调节细胞膜的组成以模拟宿主细胞膜。因此肺炎支原体易于产生慢性或潜伏性感染，而且因其细胞膜组成与人细胞相似，可能导致几种器官和组织中的自身免疫反应（如：类风湿性关节炎）。

体外培养法很少用于检测肺炎支原体，可能的检测方法包括：免疫印迹，免疫荧光染色，血细胞吸附试验，四唑还原，代谢抑制试验，血清学试验和聚合酶链式反应（PCR）等。由于成本低，测试时间相对较短，EIA血清学分析是用于诊断的最常用的肺炎支原体检测方法，其缺点在于需要使用活组织，这种取样方式可能会加大感染的严重程度。PCR是确定肺炎支原体存在的最快速和最有效的方法，有更高的灵敏度，检测特异性强，且检测时间短，仅需几个小时即可获得结果。

 

肺炎支原体PCR检测试剂盒选取了肺炎支原体细胞粘附素蛋白基因的一段序列进行PCR鉴定，引物经BLAST验证为特异性靶向肺炎支原体，与其他生物的基因组无交叉反应。使用本试剂盒检测了572个样本，得到83个阳性，经测序验证无误。可见本试剂盒具有物种特异性，可用于肺炎支原体的鉴定和检测。

 

 本试剂盒仅供科研使用。

 

 

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