Store at -20°C in the dark and avoid repeated freezing and thawing when transported at 2-8°C.

Without repeated freezing and thawing, the shelf life is one year.

 

Kit features:

 

1, can be used as a universal test kit for haemophilic mycoplasma, which can detect Most of the currently known haemophilic mycoplasmas.

 

2. Use hot-start Taq enzyme to Inhibit non-specific amplification and reduce background fluorescence.

 

3, with positive control sample, can be used to test the effectiveness of the kit and verify whether the sample to be tested contains PCR inhibitors.

 

4, with ROX reference dye, help Correct for loading errors and tube-to-tube differences.

 

5, with UDG enzyme and dUTP, can Reduce residual DNA contamination.

 

6. This kit has high sensitivity and can detect At least 1-10 genomes should be removed from the reaction solution.

 

Haemotropic mycoplasmas parasitizes in the blood of a variety of mammals, attaches to the surface of red blood cells, has no cell wall, is pleomorphic, and can pass through the blood spread. Haemophilus Mycoplasma was once named as Eperythrozoon and Haemobartonella genus), classified as the genus Rickettsia, has been reclassified as the genus Mycoplasma. Clinically, hemolytic mycoplasma can cause hemolytic anemia. Other symptoms include: anorexia, lethargy, dehydration, weight loss, sudden death, etc. Sometimes it manifests as an acute attack, and many animals have mild or no symptoms after infection. Haemophilic mycoplasmas infected by different species of animals have genetic similarities, suggesting the possibility of cross-species transmission. Haemophilic mycoplasma has been found in cats, dogs, pigs, cattle, sheep, camels, rats and other animals, and the blood mycoplasma infection in many animals is still unknown.


Haemophilic mycoplasma cannot be cultured in vitro. PCR method is generally used to culture it in blood or tissue. Detected in samples. Conventional PCR methods are more cumbersome, time-consuming, and more likely to produce non-specific amplification; probe method real-time quantitative PCR has stronger specificity, is less contaminated by residual DNA, and is more difficult to identify unknown species; SYBR method real-time quantification PCR can combine the advantages of both. Different PCR products can be distinguished through melting curves, which improves the specificity of detection. Compared with the probe method, it can also identify unknown species to a certain extent.


This kit uses the SYBR dye method, based on the 16S rRNA gene of Haemophilus Mycoplasma Sequence, design universal primers that can identify all known haemophilic mycoplasmas that parasitize common mammals . Verified by BLAST, this kit has no cross-reactivity with other species other than Haemophilus Mycoplasma. Non-specific signals that may be generated by individual species can be easily eliminated through the Tm value. This kit was used to test 320 cat blood samples, and 139 positive results were obtained, including all three haemophilic mycoplasma species in cats. Test the amplification productThe sequence was confirmed to be specific amplification of Mycoplasma haemophilum.

The hemophilic mycoplasmas that this kit can identify include:Mycoplasma haemofelis , Candidatus Mycoplasma haemominutum, Candidatus Mycoplasma turicensis, Mycoplasma haemocanis, Candidatus Mycoplasma haematoparvum, Mycoplasma suis, Mycoplasma wenyonii, Candidatus Mycoplasma haemobos, Mycoplasma ovis, Mycoplasma haemomuris, Mycoplasma coccoides, Candidatus Mycoplasma haemolamae, Mycoplasma erythrodelphis, Candidatus Mycoplasma kahanei, etc.

This kit contains hot-start Taq enzyme, dNTP, primers, positive controls, SYBR Green I dye, ROX dye, and UDG to prevent residual DNA contamination. Users only need to prepare DNA samples to use.


Hot-start Taq enzyme combines DNA polymerase with specific monoclonal antibodies. Polymerase activity is inhibited by antibodies at room temperature. During the denaturation stage of the PCR cycle, the antibody is removed by heat and the polymerase activity is restored, thus obtaining a "hot start" function, which can reduce the contamination of residual DNA and improve PCR amplification efficiency, sensitivity and target fragment yield.

 

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