Details
Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.
Kit features:
1. Specific identification of Candidatus Mycoplasma turicensis, and other biological genomes No cross-reactivity.
2. Use hot-start Taq enzyme to inhibit non-specific amplification and reduce background fluorescence.
3, with positive control sample, which can be used to test the effectiveness of the kit and verify the sample to be tested Does it contain PCR inhibitors?
4, with ROX reference dye to help correct sampling errors and differences between tubes.
5. With UDG enzyme and dUTP, it can reduce the contamination of residual DNA.
It is currently known to be parasitic on There are three types of mycoplasma in cat blood: Mycoplasma haemofelis), Candidatus Mycoplasma haemominutum and Candidatus Mycoplasma turicensis. Before the advent of PCR technology, these three mycoplasmas were collectively called Haemobartonella felis. It was reported that about 14% of anemic cats were positive for hematological mycoplasma. Candidatus Mycoplasma turicensis parasitizes on the surface of feline red blood cells. It has moderate pathogenicity among the above three mycoplasmas and can cause mild to acute hemolytic anemia. When co-infected with feline immunodeficiency virus, or cortisone After treatment with similar drugs, clinical symptoms worsened.
Commonly used detection methods include microscopy and PCR. The microscopy method has low sensitivity and large experimental errors. It cannot distinguish mycoplasma types and is susceptible to interference from other substances in the blood and artificial smear methods. Mycoplasma is easy to fall off from the surface of red blood cells, resulting in missed detection and may not be detected in chronic attacks. The PCR method has obvious advantages in sensitivity and can distinguish mycoplasma species. Compared with the ordinary PCR method, the quantitative PCR method can not only accurately quantify, but also be more convenient to operate and less affected by environmental pollution.
This kit designs specific primers for the conserved region of the 16S rRNA gene. It can accurately identify Candidatus Mycoplasma turicensis and has no cross-reaction with other biological genomes. 139 cat blood samples were tested and 4 positive results were obtained, 2 of which were mixed infections with other haemophilic mycoplasmas.
This kit contains hot-start Taq enzyme, dNTP, primers, positive controls, SYBR Green I dye, ROX dye, and UDG to prevent residual DNA contamination. Users only need to prepare DNA samples to use.
-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。
试剂盒特点：
1， 特异性识别Candidatus Mycoplasma turicensis,与其他生物基因组无交叉反应。
2， 使用热启动的Taq酶，可抑制非特异性扩增，降低背景荧光。
3， 带有阳性对照样品（组分C），可用于检验试剂盒有效性，以及验证待测样品中是否含有PCR抑制剂。
4， 带有ROX参比染料，帮助校正加样误差和管间差异。
5， 带有UDG酶和dUTP，可降低残留DNA的污染。
目前已知寄生于猫血中的支原体有三种：猫血支原体（Mycoplasma haemofelis），Candidatus Mycoplasma haemominutum和Candidatus Mycoplasma turicensis。在PCR技术出现之前，这三种支原体被统称为猫血巴通氏体（Haemobartonella felis），有报道约14%的贫血猫呈血液支原体阳性。Candidatus Mycoplasma turicensis寄生于猫红细胞表面，在上述三种支原体中致病能力中等，可引起温和到急性的溶血性贫血，与猫免疫缺陷病毒合并感染时，或者可的松类药物处理后，临床症状加重。
常用的检测方法包括显微镜镜检法和PCR法。镜检法灵敏度低，实验误差大，无法区分支原体种类，易受血液中其他物质以及人工涂片方法的干扰，且支原体易从红细胞表面脱落，造成漏检，在慢性发作时可能无法检测到。而PCR法在灵敏度上则有明显的优势，且可以区分支原体种类。定量PCR法与普通PCR法相比，不仅可以精确定量，而且操作更为方便，更少受环境污染的影响。
本试剂盒针对16S rRNA基因的保守区域，设计特异性引物，可准确识别Candidatus Mycoplasma turicensis,与其他生物基因组无交叉反应。检测139个猫血样本，得到4个阳性，其中2个为与其他嗜血支原体混合感染。
本试剂盒包含热启动Taq酶、dNTP（以UTP代替了TTP）、引物、阳性对照品、SYBR Green I染料、ROX染料，和用以防止残余DNA污染的UDG（尿嘧啶-N-糖苷酶），用户只需准备好DNA样品即可使用。
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