Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.

 

 

Kit Features:

1. Specific detection of Mycoplasma california without cross-reaction with other organisms.

2. High sensitivity, the detection limit is equivalent to about 1 copy in the reaction solution.

3. The DNA polymerase used has the characteristics of strong anti-inhibition ability and good thermal stability; using hot start method, it can Inhibit non-specific amplification and reduce background fluorescence.

4, with positive control sample, which can be used to test the effectiveness of the kit.

5. With UDG enzyme and dUTP, it can reduce the contamination of residual DNA.

Mycoplasma california is a pathogenic bovine parasitic mycoplasma second only to Mycoplasma bovis in popularity. It can cause mastitis, arthritis, pneumonia and other diseases. It was first discovered in 1977 Isolated in California, USA. In addition to the breast, Mycoplasma california can also be isolated from the uterus, lungs, inflamed joints, etc. Compared with Mycoplasma bovis, the mastitis caused by it is more contagious, the symptoms are more severe, and it is not sensitive to antibiotics. If it is not isolated in time, it will easily cause greater economic losses.


Traditional mycoplasma detection uses in vitro culture methods. In vitro culture conditions are harsh and time-consuming. It is longer, usually 7-10 days, the operation is cumbersome, and specific methods are required for identification. Due to the slow growth, it is easily interfered by other bacteria. Highly sensitive and specific PCRThe method can effectively overcome these shortcomings. Its detection time is short and the results can be obtained in just a few hours. Compared with the ordinary PCR method, the quantitative PCR method can not only accurately quantify, but also be more convenient to operate and less affected by environmental pollution.


This kit selects the RNA polymerase gene as the target and specifically identifies California Mycoplasma does not cross-react with the genomes of other organisms. 16 species of mycoplasma and 20 species of common bacteria with similar distribution ranges were detected, and no false positive results were found. 281 samples were tested and 23 positive samples were obtained, which was completely consistent with the gene sequencing results. It can be seen that this kit has very good specificity.


This kit contains hot-start DNA polymerase, dNTP, primers, probes, positive controls, ROX dye, and UDG to prevent residual DNA contamination. Users only need to prepare DNA samples and use them.


The DNA polymerase used in this kit is derived from extremothermophilic bacteria, which has been modified to have stronger resistance to inhibition and thermal stability than ordinary Taq enzymes. By binding specific monoclonal antibodies, DNA polymerase activity is inhibited at room temperature. During the thermal denaturation phase of the PCR cycle, the antibodies are heated and removed, and the DNA polymerase activity is restored at this time, thus obtaining the "hot start" function. Hot start can reduce the contamination of residual DNA and improve PCR amplification efficiency, sensitivity and target fragment yield.

 

 

-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。

 

 

试剂盒特点：

1， 特异性检测加利福尼亚支原体，与其他生物没有交叉反应。

2， 灵敏度高，检测极限相当于反应液中约含1个拷贝。

3， 使用的DNA聚合酶具有抗抑制能力强和热稳定性好的特点；采用热启动方式，可抑制非特异性扩增，降低背景荧光。

4， 带有阳性对照样品（组分C），可用于检验试剂盒有效性。

5， 带有UDG酶和dUTP，可降低残留DNA的污染。

 

加利福尼亚支原体（Mycoplasma Californicum）是流行性仅次于牛支原体（Mycoplasma Bovis）的致病性牛寄生支原体，可引起乳腺炎、关节炎、肺炎等疾病，最初于1977年在美国加利福尼亚分离得到。除了乳腺之外，在子宫、肺、发炎的关节等处也可以分离得到加利福尼亚支原体。其引起的乳腺炎与牛支原体相比，传染性更强，症状更为严重，且对抗生素不敏感，如果没有及时隔离的话容易引起较大的经济损失。

传统的支原体检测采用体外培养法，体外培养条件较为苛刻，用时较长，一般需要7-10天时间，操作较为繁琐，并且需要特定的方法来进行鉴定，由于生长缓慢，所以容易受到其他细菌的干扰。高灵敏度和高特异性的PCR法可以有效克服这些缺点，其检测时间短，仅需几个小时即可获得结果。定量PCR法与普通PCR法相比，不仅可以精确定量，而且操作更为方便，更少受环境污染的影响。

本试剂盒选择RNA聚合酶基因作为靶点，特异性识别加利福尼亚支原体，与其他生物基因组不产生交叉反应。检测分布范围相似的16种支原体和20种常见细菌，未见假阳性结果。对281个样本进行检测，获得23个阳性样本，与基因测序结果完全一致，可见本试剂盒具有非常好的特异性。

本试剂盒包含热启动DNA聚合酶、dNTP（以dUTP代替dTTP）、引物、探针、阳性对照品、ROX染料，和用以防止残余DNA污染的UDG（Uracil- DNA Glycosylase，尿嘧啶-DNA-糖基酶），用户只需准备好DNA样品即可使用。

本试剂盒采用的DNA聚合酶源自极端嗜热菌（Thermus thermophilus），经改造后较普通的Taq酶具有更强的抗抑制能力和热稳定性。通过结合特异性单克隆抗体，在室温下DNA聚合酶活性被抑制。在PCR循环的热变性阶段，抗体受热被去除，此时DNA聚合酶活性恢复，因此获得“热启动”功能。热启动可减少残余DNA的污染，提高PCR扩增效率、灵敏度和目的片段产量。

 

 

 

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