Details
Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.
Features of the kit:
1. Specifically identifies Mycoplasma ovis pneumoniae and has no cross-reaction with the genomes of other organisms.
2. High sensitivity, the lowest detection limit is 22 genomes in the reaction solution.
3. Use hot-start DNA polymerase to inhibit non-specific amplification and reduce background fluorescence.
4, with positive control sample, which can be used to test the effectiveness of the kit.
5, with ROX reference dye to help correct sampling errors and differences between tubes.
6, with UDG enzyme and dUTP, which can reduce the contamination of residual DNA.
Mycoplasma ovis pneumonia is a respiratory pathogen that can infect sheep and can cause sheep, goats and some wild small ruminants Mycoplasma sheep pneumonia , while increasing the susceptibility of animals to other pathogens , causing the condition to become more severe. The pathogenesis of Mycoplasma ovis involves several aspects, such as: changing macrophage activity, inducing the ciliated epithelium of ruminants to produce suicide antibodies, inhibiting lymphocyte activity, etc. These are all important factors leading to diseases in sheep and other small ruminants. . M. ovis can also serve as a predictor of other bacterial and viral infections. in a movingDifferent strains of M. ovis are often found in animals, and different strains differ in their virulence. Mycoplasma ovis can be isolated from the lungs, trachea and nasal cavity of small ruminants, and detection can be performed by in vitro culture, PCR and serology.
Mycoplasma ovis pneumoniae is difficult to culture in vitro, the operation is cumbersome, and the growth is slow. It takes about Within two weeks, it is often covered up by other faster-growing mycoplasmas. Serological methods have low detection sensitivity. The PCR method has the advantages of fast speed, strong specificity and high sensitivity. It is a rapid and powerful detection method, and its detection sensitivity is similar to or slightly higher than that of mycoplasma in vitro culture. Ordinary PCR experiments are cumbersome and cannot quantify target DNA. In contrast, real-time quantitative PCR can not only quantitatively analyze target DNA, but the experimental steps are also simpler and more sensitive.
This kit selects the adhesin protein gene as the target, and the primers are verified by BLAST Specifically targets Mycoplasma ovis pneumoniae. This kit was used to detect 9 types of mycoplasma and 4 types of bacteria with similar distribution ranges, and there was no non-specific reaction; 126 swabs or animal tissue samples were tested, 79 were positive, and ten PCR products were randomly selected for sequencing, confirming Mycoplasma ovis pneumonia. It can be seen that this kit has good specificity.
-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。
试剂盒特点：
1， 特异性识别绵羊肺炎支原体，与其他生物基因组没有交叉反应。
2， 灵敏度高，最低检测极限为反应液中22个基因组。
3， 使用热启动的DNA聚合酶，可抑制非特异性扩增，降低背景荧光。
4， 带有阳性对照样品（组分C），可用于检验试剂盒有效性。
5， 带有ROX参比染料，帮助校正加样误差和管间差异。
6， 带有UDG酶和dUTP，可降低残留DNA的污染。
绵羊肺炎支原体（Mycoplasma ovipneumoniae）是一种可以感染羊的呼吸道病原体，可以在绵羊、山羊以及一些野生小型反刍类动物中引起羊支原体肺炎（又称羊传染性胸膜肺炎、非进行性肺炎），同时增加动物对其他病菌的易感性，导致病情变重。绵羊肺炎支原体的致病机制涉及几个方面，如：改变巨噬细胞活性、诱导反刍动物的纤毛上皮产生自杀抗体、抑制淋巴细胞活性等，这些都是导致绵羊和其他小反刍动物疾病的重要因素。绵羊肺炎支原体还可以作为其他细菌和病毒感染的预测因子。在一只动物中经常发现不同的绵羊肺炎支原体菌株，不同的菌株在毒力方面也不同。 绵羊肺炎支原体可以在小型反刍动物的肺，气管和鼻腔内分离出来，检测可以通过体外培养、PCR和血清学等方法进行。
绵羊肺炎支原体较难体外培养，操作繁琐，生长慢，大约需要两周的时间，常有其他生长较快的支原体将其掩盖。血清学方法检测灵敏度较低。PCR法具有速度快、特异性强、灵敏度高的优势，是一种快速而强大的检测手段,与支原体体外培养的检测敏感度相似或略强。普通PCR实验操作较为繁琐，无法对目标DNA进行定量，相比之下，实时定量PCR不仅可以对目标DNA进行定量分析，实验步骤也较为简单，且灵敏度更高。
本试剂盒选取黏附素蛋白基因作为靶点，引物经BLAST验证为特异性靶向绵羊肺炎支原体。使用本试剂盒检测了分布范围类似的9种支原体和4种细菌，均无非特异性反应；检测126个拭子或动物组织样本，79个为阳性，随机选取其中十个PCR产物进行测序，证实为绵羊肺炎支原体。可见本试剂盒具有良好的特异性。
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