Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.

 

 

Kit Features:

1. Specific detection of Mycoplasma in porcine synovial fluid, without cross-reaction with other biological genomes.

2. High sensitivity, the lowest detection limit is 10 copies in the reaction solution.

3. Use hot-start Taq enzyme to inhibit non-specific amplification and reduce background fluorescence.

4, with positive control sample, which can be used to test the effectiveness of the kit.

5, with dUTP and UDG enzymes, which can reduce the contamination of residual DNA.

 

Mycoplasma porcine synovial fluid is widely distributed in pigs around the world and is easily transmitted in infected pigs. It is excreted through respiration, saliva and feces and spreads rapidly in the form of droplets, sometimes without causing clinical symptoms. Sometimes serious illness can result. Mycoplasma suis synoviae is a commensal of the tonsils and upper respiratory tract of many pigs, and these pigs may become lifelong carriers. Mycoplasma suis synovialis can circulate in the blood and has an affinity for joint tissue, infecting joints and tendon sheaths, causing non-suppurative arthritis, resulting in obvious lameness and pain. It is easy to cause disease in growing pigs aged 8-30 weeks, but it is very rare in older sows because the immune resistance of the pig body has already formed. This immune resistance can be passed to offspring through colostrum. and gradually disappear over several weeks.

Both in vitro culture method and PCR method can be used to detect Mycoplasma in porcine synovial fluid. Compared with culture methods and serological detection methods, PCR methods are fast, specific and sensitive.Advantage. Ordinary PCR experiments are cumbersome and cannot quantify target DNA. In contrast, real-time quantitative PCR can not only quantitatively analyze target DNA, but the experimental steps are also simpler and more sensitive.

Probe method porcine synovial fluid Mycoplasma quantitative PCR kit selects RNA polymerase β The subunit gene is used as a target and has been verified by BLAST to specifically target Mycoplasma porcine synoviae and has no cross-reactivity with other biological genomes. This kit was used to detect 12 types of porcine respiratory bacteria and viruses, and no cross-reaction occurred. This kit is suitable for the detection and identification of Mycoplasma in porcine synovial fluid.

 

 

-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。

 

 

试剂盒特点：

1， 特异性检测猪滑液支原体，与其他生物基因组无交叉反应。

2， 灵敏度高，最低检测极限为反应液中10个拷贝。

3， 使用热启动的Taq酶，可抑制非特异性扩增，降低背景荧光。

4， 带有阳性对照样品（组分C），可用于检验试剂盒有效性。

5， 带有dUTP和UDG酶，可降低残留DNA的污染。

 

猪滑液支原体（Mycoplasma Hyosynoviae）在全世界的猪群中广泛分布，易于在感染的猪体内传播，通过呼吸、唾液和粪便排出体外，以液滴形式迅速传播，有时不引起临床症状，有时会导致严重的疾病。猪滑液支原体是许多猪的扁桃体和上呼吸道共生菌，这些猪可能成为其终生携带者。猪滑液支原体可以在血液中循环，对关节组织有亲和力，感染关节和肌腱鞘，引起非化脓性关节炎（猪滑液支原体关节炎），导致明显的跛行和疼痛。在年龄在8-30周龄的生长猪身上易引起疾病，在较老的母猪中非常罕见，因为猪体对其产生的免疫抵抗已经形成，这种免疫抵抗可通过初乳传给后代，并在数周内逐渐消失。

体外培养法和PCR法均可用于检测猪滑液支原体。与培养法和血清学检测方法相比，PCR法具有速度快、特异性强、灵敏度高的优势。普通PCR实验操作较为繁琐，无法对目标DNA进行定量，相比之下，实时定量PCR不仅可以对目标DNA进行定量分析，实验步骤也较为简单，且灵敏度更高。

探针法猪滑液支原体定量PCR试剂盒选取RNA聚合酶β亚基基因作为靶点，经BLAST验证为特异性靶向猪滑液支原体，与其他生物基因组无交叉反应。使用本试剂盒检测12种猪呼吸道细菌和病毒，均无交叉反应发生。本试剂盒适用于猪滑液支原体的检测和鉴定。

 

 

Cartpieces

• 
• 1

  1Delete

• 
• 6

  6Delete

• 
• q

  qDelete

• 
• P

  PDelete

• 
• +

  +Delete

• 
• 2

  2Delete

• 
• 2

  2Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• P

  PDelete

• 
• �

  �Delete

• 
• <

  <Delete

• 
• i

  iDelete

• 
• i

  iDelete

• Delete

• 
• 1

  1Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• Delete

• 
• 2

  2Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• Delete

• 
• -

  -Delete

• 
• -

  -Delete

• 
• 0

  0Delete

• Delete

• Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• f

  fDelete

• Delete

• Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• 
• S

  SDelete

• Delete

• Delete

• 
• 1

  1Delete

• 
• $

  $Delete

• Delete

• 
• 0

  0Delete

• Delete

• Delete

• Delete

• 
• b

  bDelete

• 
• P

  PDelete

• 
• 5

  5Delete

• 
• P

  PDelete

Totalgoods,subtotals:￥Checkout