Store at -20°C in the dark and avoid repeated freezing and thawing when transported at 2-8°C. Without repeated freezing and thawing, the shelf life is one year.

 

Common parasitism in pigs Mycoplasmas include the following: Mycoplasma suis, Mycoplasma hyopneumoniae, Mycoplasma floccule , Mycoplasma hyorhinis and Mycoplasma hyosynoviae . Mycoplasma suis, also known as Eperythrozoon Suis, is classified as a haemophilic mycoplasma.

 

Hemotropic Mycoplasma is a type of bacteria that lives on the surface of red blood cells and has no cell wall. It was once named Hemobartonella and Eperythrozoon, was reclassified as a mycoplasma based on 16S rRNA gene sequence analysis. There are three types of haemophilic mycoplasmas that have been found in pigs: Mycoplasma suis, Mycoplasma parvum and Candidatus Mycoplasma haemosuis .

 

Mycoplasma suis was formerly known as Eperythrozoon suis), first reported in 1932, is rod-shaped, pleomorphic, and is the causative agent of porcine eperythrozoidosis. Symptoms after pig infection are diverse. During an acute attack, sows will show symptoms such as anemia, jaundice, and anorexia for 1-3 days, which may be accompanied by fever, decreased milk production, and abnormal maternal behavior; piglets will show severe anemia and fever. . After the acute attack, it enters the chronic infection stage, which is characterized by slow growth, susceptibility to disease, and decreased reproductive capacity. Experiments have shown that pig branchThe pathogen is directly related to reproductive problems in sows.

 

There is no suitable in vitro culture method for Mycoplasma suis, and the detection method is generally Giemsa. Stained blood smear method. The detection sensitivity and specificity of the blood smear method are relatively poor, and it is easily interfered by the preparation method and other components in the blood. In the acute attack stage, the bacteria are not yet obvious and cannot be detected until the disease progresses further. Serological methods are rarely used due to difficulty in obtaining sufficient antigens. The PCR method is more convenient and more sensitive, and the detection time is short, and the results can be obtained in just a few hours.

 

This kit performs PCR identification of the 16S rRNA gene, and the primers have been verified by BLAST as It specifically targets Mycoplasma suis and has cross-reactivity with Candidatus Mycoplasma haematoparvum in dogs. It has weak cross-reactivity with Haemophilus mycoplasma in other animals, but has no cross-reactivity with Mycoplasma parvum. Interference from these cross-reactions can be avoided by strict procedures when performing blood sampling. Testing using blood DNA samples from 6 pigs showed that this kit will not be interfered by Mycoplasma parvum and other microorganisms. This kit can be used for the identification and detection of Mycoplasma suis. When using it, care must be taken not to contaminate the blood products of other animals.

This product is for scientific research only.

 

 

 

 

-20℃避光保存，2-8℃运输避免反复冻融。没有反复冻融的情况下，保质期一年。

 

 

常见的寄生于猪的支原体有如下几种：猪支原体（Mycoplasma suis），猪肺炎支原体（Mycoplasma hyopneumoniae），絮状支原体（Mycoplasma flocculare），猪鼻支原体（Mycoplasma hyorhinis）和猪滑液支原体（Mycoplasma Hyosynoviae）。猪支原体也称为猪附红细胞体（Eperythrozoon Suis），在分类上属于嗜血支原体。

嗜血支原体（Hemotropic Mycoplasma，或hemoplasma)是一类寄生于红细胞表面的细菌，无细胞壁，曾被命名为血巴通氏体（Hemobartonella）和附红细胞体（Eperythrozoon），根据16S rRNA基因序列分析，被重新归类为支原体。在猪体内已发现的嗜血支原体有三种：猪支原体、小支原体（Mycoplasma parvum）和Candidatus Mycoplasma haemosuis（存在与否尚存争议）。

猪支原体旧称猪附红细胞体（Eperythrozoon suis），最早报道于1932年，呈杆状，具多形性，是猪附红细胞体病的病原体。猪感染后症状表现多样，急性发作时，母猪表现出贫血、黄疸和厌食等症状1-3天，同时可能伴随发烧和产奶量下降，以及母性行为异常；仔猪则表现为严重贫血和发烧。急性发作后进入慢性感染阶段，此时表现为生长缓慢、易患病、繁殖能力下降。有实验表明，猪支原体和母猪的生殖问题直接相关。

猪支原体尚无合适的体外培养方法，检测方法一般采用吉姆萨染色的血涂片法。血涂片法的检测灵敏度和特异性均比较差，容易受到制片方法和血液里其他成分的干扰。在急性发作期，此时菌体尚不明显，需病程进一步发展才能检测。血清学方法由于难以获得足够的抗原，所以很少得到应用。而使用PCR法检测则更为方便，且灵敏度更高，检测时间短，仅需几个小时即可获得结果。

本试剂盒针对16S rRNA基因进行PCR鉴定，引物经BLAST验证为特异性靶向猪支原体，与犬的Candidatus Mycoplasma haematoparvum有交叉反应，与另一些动物的嗜血支原体有弱交叉反应，但是和小支原体无交叉反应。在进行血液取样时严格操作即可避免这些交叉反应的干扰。使用6只猪的血液DNA样本检测表明，本试剂盒不会受到小支原体和其他微生物的干扰。本试剂盒可用于猪支原体的鉴定和检测，使用时需注意不要污染其他动物的血制品。

本产品仅供科学研究使用。

 

 

 

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