The endoplasmic reticulum is present in various eukaryotic cells except mature red blood cells of mammals. The endoplasmic reticulum is a system of interconnected lamellar gaps or tubules composed of biological membranes. The gap-like spaces between the membranes are called pools, and are usually not directly connected to the extracellular space and the cytoplasmic matrix. This membranous duct system within the cell not only constitutes a pathway for intracellular material transport, but also provides a broad reaction area for various enzyme reactions within the cell. The functions of the endoplasmic reticulum are related to protein synthesis, carbohydrate and lipid synthesis, detoxification, and assimilation, and it also has the function of transporting proteins. The Plant Endoplasmic Reticulum Membrane Protein Extraction Kit uses a simple and fast method to extract endoplasmic reticulum membrane proteins from various plant samples. The extraction process is simple and convenient, and high-quality endoplasmic reticulum membrane proteins can be extracted within two hours at the fastest. . This kit contains a unique formula that effectively dissolves membrane components. The kit contains a protease inhibitor mixture and a phosphatase inhibitor mixture, which prevents protein degradation by proteases and ensures the extraction of high-quality proteins. The protein extracted by this kit can be used for downstream protein research experiments such as Western Blotting, protein electrophoresis, immunoprecipitation, ELISA, transcription activity analysis, Gelshift gel retardation experiment, enzyme activity determination, etc. The proteins extracted by this kit are active proteins with natural protein conformation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography.

 

Applicable samples: plants

Remarks:

The above data are all from public literature and have not been independently verified by Sunlong Biotech. They are for reference only.

These protocols are for reference only. Sunlong Biotech does not independently validate these methods.

 

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