TEL: +86 571 56623320 EMAIL: SALES@SUNLONGBIOTECH.COM
Drug Names
Generic Name: Porcine Chromogranin A (CHGA) ELISA Kit
Purpose
Our Porcine Chromogranin A (CHGA) ELISA kit is to assay CHGA levels in Porcine serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to CHGA, Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for CHGA is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain CHGA and HRP conjugated CHGA antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of CHGA. You can calculate the concentration of CHGA in the samples by comparing the OD of the samples to the standard curve.
Scan Wechat Qrcode