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Summary:
Purpose
Our Quick Step Human anti-cardiolipin antibody Igm (ACA-IgM)ELISA kit is to assay ACA-IgM levels in Human serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to ACA-IgM. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)- conjugated antigen specific for ACA-IgM is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain ACA-IgM and HRP conjugated ACA-IgM antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of ACA-IgM. You can calculate the concentration of ACA-IgM in the samples by comparing the OD of the samples to the standard curve.
Typical Data
The standard curve of QS2515Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Standard
Concentration
OD Value
Average OD Value
Blank Well
0U/ml
0.046
0.0435
0.041
S1
24U/ml
1.989
1.9925
1.996
S2
16U/ml
1.331
1.3420
1.353
S3
8U/ml
0.694
0.7025
0.711
S4
4U/ml
0.391
0.3865
0.382
S5
2U/ml
0.194
0.1975
0.201
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