Summary:

 

Purpose

 

Our Quick Step Human Immunoglobulin M,IgM ELISA Kit is to assay IgM levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to IgM. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)- conjugated antigen specific for IgM is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain IgM and HRP conjugated IgM antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of IgM. You can calculate the concentration of IgM in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0917Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/ml	0.044	0.0480
		0.052
S1	60ng/ml	1.999	1.9865
		1.974
S2	40ng/ml	1.302	1.3115
		1.321
S3	20ng/ml	0.687	0.6900
		0.693
S4	10ng/ml	0.392	0.3855
		0.379
S5	5ng/ml	0.202	0.2030
		0.204

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