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Quick Step Human Oxidized Low Density  Lipoprotein antibody IgM  (OxLDL-IgM)elisa Kit
Quick Step Human Oxidized Low Density  Lipoprotein antibody IgM (OxLDL-IgM)elisa Kit
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  • NO.:QS3569Hu
    Sensitivity:0.5 μg/L
    Assay range:1.5 μg/L - 80 μg/L
    Standard:90 μg/L
    Sample Volume:10ul
    Wavelength:450nm
    Assay Time:60 minutes
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Details

 

Summary:

 

Purpose

 

Our Quick Step Human Oxidized Low Density  Lipoprotein antibody IgM (OxLDL-IgM)elisa Kit is to assay OxLDL-IgM levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to OxLDL-IgM. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)- conjugated antigen specific for OxLDL-IgM is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain OxLDL-IgM and HRP conjugated OxLDL-IgM antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of OxLDL-IgM. You can calculate the concentration of OxLDL-IgM in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS3569Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard

Concentration

OD Value

Average OD Value

Blank Well

0μg/L

0.044

0.0480

0.052

S1

60μg/L

1.999

1.9865

1.974

S2

40μg/L

1.302

1.3115

1.321

S3

20μg/L

0.687

0.6900

0.693

S4

10μg/L

0.392

0.3855

0.379

S5

5μg/L

0.202

0.2030

0.204 

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