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Summary:
Purpose
Our Quick Step Human Anti-Ribosomal P protein antibody IgG (Rib-P IgG)ELISA Kit is to assay Rib-P IgG levels in Human serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to Rib-P IgG. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)- conjugated antigen specific for Rib-P IgG is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Rib-P IgG and HRP conjugated Rib-P IgG antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rib-P IgG. You can calculate the concentration of Rib-P IgG in the samples by comparing the OD of the samples to the standard curve.
Typical Data
The standard curve of QSD4141Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Standard
Concentration
OD Value
Average OD Value
Blank Well
0pg/ml
0.044
0.0480
0.052
S1
60pg/ml
1.999
1.9865
1.974
S2
40pg/ml
1.302
1.3115
1.321
S3
20pg/ml
0.687
0.6900
0.693
S4
10pg/ml
0.392
0.3855
0.379
S5
5pg/ml
0.202
0.2030
0.204
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