Summary:

 

Purpose

 

Our Quick Step Human desmogleins 1 antibody IgG (DSG1-IgG)ELISA Kit is to assay DSG1-IgG levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to DSG1-IgG. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)- conjugated antigen specific for DSG1-IgG is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain DSG1-IgG and HRP conjugated DSG1-IgG antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of DSG1-IgG. You can calculate the concentration of DSG1-IgG in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS3390Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.044	0.0480
		0.052
S1	60pg/ml	1.999	1.9865
		1.974
S2	40pg/ml	1.302	1.3115
		1.321
S3	20pg/ml	0.687	0.6900
		0.693
S4	10pg/ml	0.392	0.3855
		0.379
S5	5pg/ml	0.202	0.2030
		0.204

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