Summary:

 

Purpose

 

Our Quick Step Human Islet Cell Antibody (ICA)elisa Kit is to assay ICA levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to ICA. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)- conjugated antigen specific for ICA is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain ICA and HRP conjugated ICA antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of ICA. You can calculate the concentration of ICA in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS1017Hu_1 is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.044	0.0480
		0.052
S1	60pg/ml	1.999	1.9865
		1.974
S2	40pg/ml	1.302	1.3115
		1.321
S3	20pg/ml	0.687	0.6900
		0.693
S4	10pg/ml	0.392	0.3855
		0.379
S5	5pg/ml	0.202	0.2030
		0.204

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