Summary:

 

Purpose

 

Our Quick Step Human Chemokine C Ligand 2 (CCL2)ELISA Kit is to assay CCL2 levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to CCL2. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for CCL2 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain CCL2 and HRP conjugated CCL2 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of CCL2. You can calculate the concentration of CCL2 in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS3287Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/L	0.035	0.0385
		0.042
S1	1200ng/L	1.973	1.9790
		1.985
S2	800ng/L	1.327	1.3330
		1.339
S3	400ng/L	0.705	0.7105
		0.716
S4	200ng/L	0.393	0.3885
		0.384
S5	100ng/L	0.186	0.1895
		0.193

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