Summary:

 

Purpose

 

Our Quick Step Rat Glutathione (GSH)ELISA Kit is to assay GSH levels in Rat serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an Antibody specific to GSH. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific Antibody. Then a Horseradish Peroxidase (HRP)- conjugated Antibody specific for GSH is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain GSH and HRP conjugated GSH Antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of GSH. You can calculate the concentration of GSH in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0998Ra is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/L	0.052	0.0530
		0.054
S1	480ng/L	1.969	1.9715
		1.974
S2	320ng/L	1.272	1.2705
		1.269
S3	160ng/L	0.785	0.7885
		0.792
S4	80ng/L	0.474	0.4785
		0.483
S5	40ng/L	0.215	0.2190
		0.223

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