Summary:

 

Purpose

 

Our Quick Step Rat Peroxiredoxin 3 (PRDX3)elisa Kit is to assay PRDX3 levels in Rat serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an Antibody specific to PRDX3. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific Antibody. Then a Horseradish Peroxidase (HRP)- conjugated Antibody specific for PRDX3 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain PRDX3 and HRP conjugated PRDX3 Antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of PRDX3. You can calculate the concentration of PRDX3 in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS1441Ra is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0单位	0.036	0.0385
		0.041
S1	600单位	1.987	1.9820
		1.977
S2	400单位	1.374	1.3780
		1.382
S3	200单位	0.733	0.7290
		0.725
S4	100单位	0.386	0.3795
		0.373
S5	50单位	0.172	0.1800
		0.188

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