Summary:

 

Purpose

 

Our Quick Step Rat Tumor necrosis factor beta,TNF-beta ELISA KIT is to assay TNF-beta levels in Rat serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to TNF-beta. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for TNF-beta is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain TNF-beta and HRP conjugated TNF-beta antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of TNF-beta. You can calculate the concentration of TNF-beta in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0723Ra is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.048	0.0505
		0.053
S1	180pg/ml	1.941	1.9370
		1.933
S2	120pg/ml	1.317	1.3255
		1.334
S3	60pg/ml	0.709	0.7200
		0.731
S4	30pg/ml	0.373	0.3645
		0.356
S5	15pg/ml	0.187	0.1900
		0.193

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