Summary:

 

Purpose

 

Our Quick Step Mouse nuclear factor-κB p65,NF-κB p65 ELISA Kit is to assay NF-κBp65 levels in Mouse serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to NF-κBp65. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for NF-κBp65 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain NF-κBp65 and HRP conjugated NF-κBp65 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of NF-κBp65. You can calculate the concentration of NF-κBp65 in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0411Mo is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.048	0.0465
		0.045
S1	18pg/ml	1.973	1.9760
		1.979
S2	12pg/ml	1.363	1.3675
		1.372
S3	6pg/ml	0.725	0.7220
		0.719
S4	3pg/ml	0.388	0.3860
		0.384
S5	1.5pg/ml	0.187	0.1895
		0.192

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