Summary:

 

Purpose

 

Our Quick Step Rat Butyrylcholinesterase (BCHE)elisa Kit is to assay BCHE levels in Rat serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to BCHE. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for BCHE is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain BCHE and HRP conjugated BCHE antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of BCHE. You can calculate the concentration of BCHE in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS1644Ra is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/ml	0.048	0.0465
		0.045
S1	18ng/ml	1.973	1.9760
		1.979
S2	12ng/ml	1.363	1.3675
		1.372
S3	6ng/ml	0.725	0.7220
		0.719
S4	3ng/ml	0.388	0.3860
		0.384
S5	1.5ng/ml	0.187	0.1895
		0.192

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