Summary:

 

Purpose

 

Our Quick Step Human Coxsackie virus B IgM, Cox V-IgM ELISA Kit (Quantitative) is to assay CoxV-IgM levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to CoxV-IgM. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)- conjugated antigen specific for CoxV-IgM is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain CoxV-IgM and HRP conjugated CoxV-IgM antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of CoxV-IgM. You can calculate the concentration of CoxV-IgM in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS3283Hu_1 is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/L	0.059	0.0575
		0.056
S1	15ng/L	1.967	1.9730
		1.979
S2	10ng/L	1.385	1.3895
		1.394
S3	5ng/L	0.706	0.7110
		0.716
S4	2.5ng/L	0.382	0.3865
		0.391
S5	1.25ng/L	0.187	0.1920
		0.197

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