Summary:

 

Purpose

 

Our Quick Step Human Coll2-1 ELISA Kit is to assay Coll2-1 levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Coll2-1. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for Coll2-1 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Coll2-1 and HRP conjugated Coll2-1 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Coll2-1. You can calculate the concentration of Coll2-1 in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QSD3928Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0μg/L	0.059	0.0575
		0.056
S1	15μg/L	1.967	1.9730
		1.979
S2	10μg/L	1.385	1.3895
		1.394
S3	5μg/L	0.706	0.7110
		0.716
S4	2.5μg/L	0.382	0.3865
		0.391
S5	1.25μg/L	0.187	0.1920
		0.197

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