Summary:

 

Purpose

Our Quick Step Rat Blood Urea Nitrogen (BUN)ELISA Kit is to assay BUN levels in Rat serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to BUN. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for BUN is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain BUN and HRP conjugated BUN antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of BUN. You can calculate the concentration of BUN in the samples by comparing the OD of the samples to the standard curve.

Typical Data

 

The standard curve of QS1053Ra is provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0nmol/ml	0.047	0.0495
		0.052
S1	4800nmol/ml	1.975	1.9690
		1.963
S2	3200nmol/ml	1.315	1.3210
		1.327
S3	1600nmol/ml	0.716	0.7110
		0.706
S4	800nmol/ml	0.359	0.3660
		0.373
S5	400nmol/ml	0.189	0.1950
		0.201

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