Summary:

 

Purpose

 

Our Quick Step Rat Free Thyroxine,FT4 ELISA Kit is to assay FT4 levels in Rat serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to FT4. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for FT4 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain FT4 and HRP conjugated FT4 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of FT4. You can calculate the concentration of FT4 in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0295Ra is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.063	0.0580
		0.053
S1	12pg/ml	1.955	1.9585
		1.962
S2	8pg/ml	1.288	1.2920
		1.296
S3	4pg/ml	0.704	0.7080
		0.712
S4	2pg/ml	0.387	0.3965
		0.406
S5	1pg/ml	0.196	0.2000
		0.204

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