Summary:

 

Purpose

 

Our Quick Step Rat pigment epithelium-derived factor,PEDF ELISA Kit is to assay PEDF levels in Rat serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to PEDF. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for PEDF is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain PEDF and HRP conjugated PEDF antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of PEDF. You can calculate the concentration of PEDF in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS0573Ra is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/ml	0.063	0.0580
		0.053
S1	12ng/ml	1.955	1.9585
		1.962
S2	8ng/ml	1.288	1.2920
		1.296
S3	4ng/ml	0.704	0.7080
		0.712
S4	2ng/ml	0.387	0.3965
		0.406
S5	1ng/ml	0.196	0.2000
		0.204

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