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Drug Names
Generic Name: Human 25-Hydroxyvitamin D (HVD)ELISA Kit
Purpose
Our Human 25-Hydroxyvitamin D (HVD)ELISA kit is to assay HVD levels in Human serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to HVD. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for HVD is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain HVD and HRP conjugated HVD antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of HVD. You can calculate the concentration of HVD in the samples by comparing the OD of the samples to the standard curve.
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