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Drug NamesGeneric Name:Canine rabies virus antibody (RV-Ab) ELISA KitPurposeOur Canine rabies virus antibody(RV-Ab) ELISA kit is to assay RV-Ab levels in Canineserum, plasma, culture media or any biological fluid.PrincipleThis ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate providedin this kit has been pre-coated with an antigen specific to RV-Ab. Standards or samples areadded to the appropriate Microelisa stripplate wells and combined to the specific antigen.Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for RV is added to eachMicroelisa stripplate well and incubated. Free components are washed away. The TMBsubstrate solution is added to each well. Only those wells that contain RV-Ab and HRPconjugated RV antigen will appear blue in color and then turn yellow after the addition of thestop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of450 nm. The presence of RV-Ab is determined by comparing with the CUTOFF value.
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