Summary:

 

Purpose

Our Quick Step Rat Uncoupling Protein 1 (UCP1)ELISA Kit is to assay UCP1 levels in Rat serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to UCP1. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for UCP1 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain UCP1 and HRP conjugated UCP1 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of UCP1. You can calculate the concentration of UCP1 in the samples by comparing the OD of the samples to the standard curve.

Typical Data

The standard curve of QS1232Ra is provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.059	0.0560
		0.053
S1	360pg/ml	1.975	1.9695
		1.964
S2	240pg/ml	1.388	1.3800
		1.372
S3	120pg/ml	0.705	0.6995
		0.694
S4	60pg/ml	0.383	0.3800
		0.377
S5	30pg/ml	0.197	0.2010
		0.205

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