Summary:

 

Purpose

Our Quick Step Rat Nitric Oxide (NO)ELISA Kit is to assay NO levels in Rat serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to NO. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for NO is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain NO and HRP conjugated NO antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of NO. You can calculate the concentration of NO in the samples by comparing the OD of the samples to the standard curve.

Typical Data

The standard curve of QS0531Ra is provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/ml	0.056	0.0540
		0.052
S1	30ng/ml	1.995	1.9890
		1.983
S2	20ng/ml	1.425	1.4195
		1.414
S3	10ng/ml	0.752	0.7455
		0.739
S4	5ng/ml	0.415	0.4085
		0.402
S5	2.5ng/ml	0.201	0.1975
		0.194

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