Summary:

 

Purpose

Our Quick Step Rat Antidiuretic Hormone (ADH)ELISA Kit is to assay ADH levels in Rat serum, plasma, culture media or any biological fluid.

 

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to ADH. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for ADH is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain ADH and HRP conjugated ADH antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of ADH. You can calculate the concentration of ADH in the samples by comparing the OD of the samples to the standard curve.

Typical Data

The standard curve of QS1549Ra is provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0	0.042	0.0485
		0.055
S1	120	1.998	1.9855
		1.973
S2	80	1.318	1.3240
		1.330
S3	40	0.688	0.6915
		0.695
S4	20	0.393	0.3875
		0.382
S5	10	0.201	0.1950
		0.189

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