Detection Device:

HPLC

Application:

Hsphasetin (HP) is a homologue of ceruloplasmin, which catalyzes the oxidation of ferrous ions (Fe2+) to ferric ions (Fe3+), then Fe3+ binds to transferrin and participates in cellular iron release. With Fe2+ as substrate, Fe2+ is oxidized to Fe3+ under the catalysis of HP. Fe2+ forms a colored complex with phenazine, and has a characteristic absorption peak at 562 nm. The content of Fe2+ which is not oxidized is calculated, and then the content of oxidized Fe2+ is obtained. So the HP activity can be reflected by the rate at Fe2+ oxidized。

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