Pyruvate Decarboxylase (PDC) Activity Assay Kit

Note: Take two or three different samples for prediction before test.

Operation Equipment: Spectrophotometer/Microplate reader

Cat No: AK0491

Size:100T/96S

Product Description:

Pyruvate  Decarboxylase  (PDC)  exists  in  yeast  mainly,  which  is  one  of the  key  enzymes  in  ethanol fermentation.

 

PDC catalyzes pyruvate decarboxylation to form acetaldehyde. The addition of alcohol dehydrogenase (ADH) can further catalyze the reduction of aldehydes by NADH to ethanol and NAD+ . NADH has an absorbance at 340 nm but NAD+ not, the activity of PDC can be calculated by measuring decrease rate of absorption at 340 nm.

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