NAD-Malate Dehydrogenase (NAD-MDH) Activity Assay Kit 

Note: It is necessary to predict 2-3 large difference samples before the formal determination.       

 Operation Equipment: Spectrophotometer

Cat No: AK0498

Size: 50T/48S

Product Description:

Malate Dehydrogenase (MDH, EC 1. 1 . 1 .37 ) is widely exist in animal, plant, microbe and cells culture. MDH  in  mitochondria  is  one  of the  key  enzymes  of TCA  cycle,  which  catalyzes  the  formation  of oxaloacetic acid from malic acid. In contrast, MDH in serum catalyzes the formation of malic acid from oxaloacetic acid. Oxaloacetic acid is an important intermediate that connects several important metabolic pathways. MDH plays an important role in many physiological activities of cells, including mitochondrial energy  metabolism,  malic  acid-aspartic  acid  shuttle  system,  reactive  oxygen  species  metabolism  and disease resistance. According to the different coenzyme specificity, MDH is divided into NAD- dependent MDH and NADP- dependent MDH. Bacteria usually only contain NAD-MDH. NAD-MDH is distributed in the cytoplasm and mitochondria in eukaryotic cells.

 

NAD-MDH catalyzes NADH to reduce oxaloacetic acid into malic acid, which resulting in a decrease in absorption at 340nm.

 

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