Oxidized Glutathione (GSSG) Content Assay Kit

Note: Take two or three different samples for prediction before test.

Operation Equipment: Spectrophotometer/microplate reader

Catalog Number: AK0475

Size:100T/96S

Product Description

 

Oxidized    Glutathione(GSSG)    is    an    oxidized    form    of   glutathione    (GSH),    also    known    as dithioneglutathione, formed by the oxidation of two molecules of glutathione. GSSG is reduced to GSH by glutathione reductase, so most of the body is in the reduced form. The determination of GSH and GSSG content and ratio of GSH/GSSG in cells can reflect the redox status of cells. This kit utilizes reaction of glutathione and 5, 5'-dithiobis-2-nitrobenoic acid (DTNB) to produce 5-thio-2- nitrobenzoic acid. 5-thio-2- nitrobenzoic acid has the largest absorption at wavelength of 412nm, and 2-Vinylpyridine inhibit reduced glutathione in the original of samples, and then using glutathione reductase to reduce GSSG to GSH, determining the content of Oxidized Glutathione.

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