Glutathione Reductase (GR)Activity Assay Kit

Note: Take two or three different samples for prediction before test.

Operation Equipment:Spectrophotometer

Cat No: AK0480

Size: 50T/48S

Product Description:

GR is a flavor-protein oxidoreductase widely existing in eukaryotes and prokaryotes. GR catalyzes the reduction of GSSG to GSH, which is one of the key enzymes of glutathione redox cycle (GR is usually replaced by TrxR in insects). GR catalyzes the reduction of GSSG to generate GSH by NADPH, which is helpful to maintain the body's GSH/GSSG ratio. GR plays a key role in the scavenging of reactive oxygen species in oxidative stress. In addition, GR also participates in the cycle pathway of ascorbic acid and glutathione.

 

GR  catalyzes  the  reduction  of  GSSG  by  NADPH  to  produce  GSH,  at  the  same  time,  NADPH dehydrogenation produces NADP+ . NADPH has a characteristic absorption at 340 nm. On the contrary, NADP+ has no absorption peak at this wavelength. The rate of NADPH dehydrogenation is determined by measuring the rate of decrease of absorbance at 340 nm, thereby calculating GR activity.

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