Hydroxyproline (HYP) Activity Assay Kit

Note: Take two or three different samples for prediction before test.

Detection equipment: Spectrophotometer/Microplate reader

Cat No: AK0571

Size: 100T/96S

Description:

HYP is one of the main components of collagen in the body. Most of the collagen is distributed in the skin, tendon,  cartilage  and blood vessels  et  al. Therefore, the  content  of HYP is  an important index reflecting the metabolism and fibrosis degree of collagen tissue.

The sample is hydrolyzed to produce free HYP, which is further oxidized by chloramine T. The oxidized product  reacted  with  p-Dimethylaminobenzaldehyde  to  produce  red  compound  with  characteristic absorption peak at 560 nm. The content of HYP can be calculated by measuring the absorption value of sample hydrolysate at 560 nm.

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