Acetyl acetylacetic acid (ACAC) is an important component of ketone body, accounting for about 20% of the normal human ketone body, which is produced by oxidation of fatty acids.Organic acid.Normal content of acetoacetic acid is harmless to the human body, and diabetic patients have reduced the use of sugar, or when hungry metabolic disorders, the amount of fat and acetoacetic acid will accumulate.Acetate can be converted to acetone or converted to β-hydroxycoherate.

Under pH 7.0 and 37 ° C, Acac reacts under beta-hydroxybutyrate dehydrogenase (HBDH) catalysis, and NADH is oxidized into NAD +; under 1-MPMS, WST-1It can be reacted with NADH to produce water-soluble Formazan, and there is a feature absorption peak at 450 nm.By detecting the wavelength change of 450 nm, the content of the ACAC can be calculated.

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