Alkaline Protease (AKP) Activity Assay Kit

Note: Take two or three different samples for prediction before test.

Detection equipment: Spectrophotometer/microplate reader

Cat No: AK0236

Size: 100T/48S

Product Description:

AKP is a serine protease, which catalyzes the hydrolysis of protein peptide bond in alkaline condition. In addition, the enzyme can hydrolyze ester bond and amide bond. It has the function of trans ester and trans peptide. The enzyme is one of the main industrial enzymes, which is widely used in pharmaceutical, silk, food, leather and other industries.

In  alkaline  condition,  AKP  hydrolyzes  casein  to  produce  tyrosine.  In  alkaline  condition,  tyrosine reduced phosphomolybdic acid to tungsten blue. Tungsten blue has a characteristic absorption peak at 680 nm. The activity of AKP can be calculated by measuring the increasing rate of 680 nm absorbance.

 

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