Measurement Significance: Fruit 1,6-diphosphate is an important intermediate of high energy metabolites and metabolic regulators in the body. It is an important intermediate for glycolysis pathways. It is widely existing in animal and plants andIn the microbial body, the concentration of potassium ions in cells can affect the metabolism of glucose and other energy substrates, promote the release of tissue oxygen, widely used in clinical medicine preparations.

Determination principle: Adehyde enzyme catalyzed fructose 1,6-diphosphate degradation, product and DNPH condensate into a purple-red substance, with a characteristic absorption peak at 540 nm.

Required instruments, consumables: balance, low temperature centrifuge, mortar, constant temperature water bath, visible spectrophotometer / enzyme label, trace quantum-based cuvette / 96-well plate.

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