Measuring significance: Coenzyme IINADP (H) is widely present in animals, plants, microorganisms and cultured cells, NADP + and NADPH content determination can calculate NADP (NADPH + NADP +) content and NADPH /NADP + ratio, its change is closely related to the phosphate pathway and biosynthesis and antioxidant reactions.NADPH / NADP + ratio is one of the main markers of cellular oxidation, but also has important regulation in PPP pathways, biosynthesis and antioxidant metabolism.

The principle of assay: NADP + and NADPH in S product were extracted with acidic and alkaline extracts, respectively.NADPH was used to control the hydrogen peroxide of the oxide thiazole blue (MTT) by PMS, and the absorbed value was detected at 570 nm to determine the NADPH content.NADP + content was detected by using 6-phosphate dehydrogenase reduction NADP + NADPH to detect NADP + content.

Instruments, consumables, consumables: visible spectrophotometer / enzyme label, desktop centrifuge, adjustable pipette, trace quantum-based cuvette / 96-well plate, mortar, ice and distilled water.

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