Measurement:

LOX is widely present in plant tissue, catalyzed an unsaturated fatty acid oxidation reaction, causing membrane fat oxidation.It has an important role in the growth and development of plants, mature aging and the processes of the stress. Determination Principle:

LOX catalyzed hydrogen acid oxidation, oxidative product has a characteristic absorption peak at 234 nm; the increase rate of the absorbance of 234 nm is measured to calculate LOX activity.

Instruments, consumables:

mortar, ice, desktop centrifuge, UV spectrophotometer / enzyme symbol, trace quantum-based cuvette / 96-well plate, adjustable pipetteGun and distilled water.

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