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Fish 11-keto Testosterone (11-KT)elisa Kit
Fish 11-keto Testosterone (11-KT)elisa Kit
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  • NO.:SLD0108FI
    Sample Volume:10ul
    Wavelength:450nm
    Assay range:8ng/L-220ng/L
    Sensitivity:1ng/L
    Qualitative:
    Standard:270ng/L
  • Goods click count:234
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Details

Drug Names

Generic Name: Fish 11-keto Testosterone (11-KT) ELISA Kit

Purpose

Our Fish 11-keto Testosterone (11-KT) ELISA kit is to assay 11-KT levels in Fish serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to 11-KT. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for 11-KT is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain 11-KT and HRP conjugated 11-KT antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of 11-KT. You can calculate the concentration of 11-KT in the samples by comparing the OD of the samples to the standard curve.

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