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Product Name TIAF1 Chinese Name TGFB1诱导抗凋亡因子1抗体 Alias MAJN; TGFB1 induced anti apoptotic factor 1 (12 kDa TGF beta1 induced antiapoptotic factor); 12 kDa TGF-beta-1-induced antiapoptotic factor; TGFB1 induced anti apoptotic factor 1; TGFB1-induced anti-apoptotic factor 1; TIAF1_HUMAN; MAJN; SPR210; MYO18A; MYSPDZ; TGFB1 induced anti apoptotic factor 1. Research Area Cell biology Signal transduction Apoptosis transcriptional regulatory factor Immunogen Species Rabbit Clonality Polyclonal React Species Human, Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 12kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human TIAF1: 34-115/115 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail TIAF-1 (TGF Beta 1-induced antiapoptotic factor 1), also known as MYO18A, MAJN or MYSPDZ, is a TGF Beta 1-induced anti-apoptotic factor that localizes to the nucleus and functions to inhibit the cytotoxic effects of TNF? TRADD and FADD. Two isoforms of mouse TIAF-1 exist and are designated MysPDZ-Alpha and MysPDZ-Beta. MysPDZ-Alpha consists of a KE-rich region, an N-terminal PDZ domain and a prevalent myosin homologous head region, while the MysPDZ-Beta isoform lacks the KE-rich region and PDZ domain. MysPDZ-Alpha is present in most tissues and is known to co-localize with the ER-Golgi complex and with membrane ruffles and filopodia. MysPDZ-Beta is expressed specifically in hematopoietic tissues and cell lines and shows dispersed localization in the cytoplasm.
Function:
This protein inhibits the cytotoxic effects of TNF-alpha and overexpressed TNF receptor adapters TRADD, FADD, and RIP. It is involved in TGF-beta1 inhibition of IkappaB-alpha expression and suppression of TNF-mediated IkappaB-alpha degradation.
Subcellular Location:
Nuclear
Tissue Specificity:
Not detectable in normal kidney and liver. Up-regulated in chronic and acute allograft rejection: expressed in the inflammatory infiltrate and in tubular epithelial cells.
SWISS:
O95411
Gene ID:
9220
Database links:Entrez Gene: 9220 Human
Omim: 609517 Human
SwissProt: O95411 Human
Unigene: 462590 Human
Product Picture Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TIAF1 Polyclonal Antibody, Unconjugated(SL7602R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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