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Product Name phospho-IKKi (Thr501) Chinese Name 磷酸化核因子NFκB抑制蛋白激酶i抗体 Alias IKKi/IKKe (phospho T501); IKKi/IKKe (phospho Thr501); p-IKKE (Thr501); p-IKKE (T501); IKKi/IKKe; I kappa B kinase epsilon; IkBKE; IKBKE protein; IKK E; IKK i; IKK-i; IKK related kinase epsilon; IKKE; IKK-E; IKKepsilon; IKKI; Inducible I kappa B kinase; inducible IkappaB kinase; Inhibitor of kappa light polypeptide gene enhancer in B cells kinase epsilon; Inhibitor of nuclear factor kappa B kinase subunit epsilon; KIAA0151; MGC125294; MGC125295; MGC125297; IKKE_HUMAN; I-kappa-B kinase epsilon; IKK-epsilon; Inducible I kappa-B kinase. Product Type Phosphorylated anti Research Area Tumour Signal transduction Apoptosis Cyclin Kinases and Phosphatases Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Cow, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 80kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthesised phosphopeptide derived from human IKKi/IKKe around the phosphorylation site of Thr501: LR(p-T)LA Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail IKBKE is a noncanonical I-kappa-B (see MIM 164008) kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer (MIM 114480) oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines (Hutti et al., 2009 [PubMed 19481526]).[supplied by OMIM, Oct 2009].
Function:
Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response. Protects cells against DNA damage-induced cell death.
Subunit:
May interact with MAVS/IPS1. Interacts with AZI2. Interacts with SIKE1. Interacts with TICAM1/TRIF, IRF3 and DDX58/RIG-I, interactions are disrupted by the interaction between IKBKE and SIKE1. Interacts with TOPORS; induced by DNA damage. Interacts with CYLD.
Subcellular Location:
Cytoplasm. Nucleus. Nucleus, PML body. Note=Targeting to PML nuclear bodies upon DNA damage is TOPORS-dependent.
Tissue Specificity:
Highly expressed in spleen followed by thymus, peripheral blood leukocytes, pancreas, placenta. Weakly expressed in lung, kidney, prostate, ovary and colon.
Post-translational modifications:
Autophosphorylated and phosphorylated by IKBKB/IKKB.
Sumoylation by TOPORS upon DNA damage is required for protection of cells against DNA damage-induced cell death. Desumoylated by SENP1.
Similarity:
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
Contains 1 protein kinase domain.
SWISS:
Q14164
Gene ID:
9641
Database links:Entrez Gene: 9641 Human
Omim: 605048 Human
SwissProt: Q14164 Human
Unigene: 321045 Human
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