Product Name	BCL2L15
Chinese Name	BCL2样15促凋亡蛋白抗体
Alias	B2L15_HUMAN; Bcl-2-like protein 15; Bcl2-L-15; BCL2-like 15; Bcl2l15; Bfk; C1orf178; FLJ22588; pro-apoptotic Bcl-2 protein.
literatures	Specific References  (1)     |     SL7582R has been referenced in 1 publications. [IF=3.759] Diqi Yanget al. BCL2L15 Depletion Inhibits Endometrial Receptivity via the STAT1 Signaling Pathway. Genes (Basel) . 2020 Jul 17;11(7):816.  WB, IF ;  Goat.   PubMed:32708974
Research Area	Cell biology  Apoptosis
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Mouse, Rat, Dog, Cow, Horse, Rabbit, Sheep, )
Applications	WB=1:200-1000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	18kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human BCL2L15: 65-163/163
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	BCL2L15 is a 163 amino acid protein and novel member of the Bcl-2 family that contains both BH2 and BH3 regions, but not BH1, BH4 or a C-terminal hydrophobic membrane anchor. Like Bcl-GL, Bfk does not bind to any Bcl-2 family members, even though its BH3 motif can mediate association with prosurvival proteins. Bfk localizes to cytoplasm, but unlike Bcl-GL, Bfk does not associate with organelles. Existing as four alternatively spliced isoforms, the pro-apoptotic isoforms of Bfk may help to protect against the development of human gastrointestinal malignancy. Bfk is found at low levels in stomach, ovary, bone marrow and spleen, but Bfk is highly expressed in mammary gland during pregnancy, suggesting that Bfk may play a role in mammary development. Bfk appears to be a cytosolic protein whose gene maps to chromosome 1p13.2. Chromosome 1 is the largest human chromosome spanning about 260 million base pairs and making up 8% of the human genome. SWISS: Q5TBC7 Gene ID: 440603 Database links: UniProtKB/Swiss-Prot: Q5TBC7.1 Entrez Gene: 440603 Human SwissProt: Q5TBC7 Human Unigene: 123106 Human
Product Picture	Sample: Lane 1: Human SW480 cell lysates Lane 2: Human MCF-7 cell lysates Lane 3: Human A549 cell lysates Primary: Anti-BCL2L15 (SL7582R) at 1/200 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18kDa Observed band size: 18 kDa Sample: Raji(Human) Cell Lysate at 30 ug Primary: Anti-BCL2L15 (SL7582R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 19kD Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BCL2L15) Polyclonal Antibody, Unconjugated (SL7582R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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