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Product Name AT2A1/SERCA1 ATPase Chinese Name 肌浆网钙ATP酶1/内质网钙ATP酶1抗体 Alias fast twitch skeletal muscle isoform; AT2A1_HUMAN; ATP2A; ATP2A1; ATPase Ca++ transporting cardiac muscle fast twitch 1; ATPase Ca++ transporting fast twitch 1; ATPase, Ca(2+)-transporting fast twitch 1; Calcium pump 1; Calcium transporting ATPase sarcoplasmic reticulum type fast twitch skeletal muscle isoform; Calcium-transporting ATPase sarcoplasmic reticulum type; EC 3.6.3.8; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; Fast skeletal muscle SR calcium ATPase; OTTHUMP00000162561; OTTHUMP00000162562; Sarcoendoplasmic reticulum calcium ATPase; Sarcoplasmic reticulum Ca(2+)-ATPase 1; Ca-ATPpase; Sarcoplasmic/endoplasmic reticulum calcium ATPase 1; SERCA 1; SERCA1; SERCA1 truncated isoform, included; SR Ca(2+) ATPase 1; SR Ca(2+)-ATPase 1. Research Area Cardiovascular Cell biology Signal transduction transcriptional regulatory factor Channel protein Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Mouse, Dog, Cow, Horse, Rabbit, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 110kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human SERCA1 ATPase: 101-200/1001 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.
Function:
This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.
Subunit:
Associated with sarcolipin (SLN) and phospholamban (PLN) (By similarity).
Subcellular Location:
Endoplasmic reticulum membrane; Multi-pass membrane protein. Sarcoplasmic reticulum membrane; Multi-pass membrane protein.
Tissue Specificity:
Skeletal muscle, fast twitch muscle (type II) fibers.
DISEASE:
Defects in ATP2A1 are the cause of Brody myopathy (BRM) [MIM:601003]. An autosomal recessive myopathy characterized by increasing impairment of relaxation of fast twist skeletal muscle during exercise.
Similarity:
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.
SWISS:
O14983
Gene ID:
487
Database links:
Entrez Gene: 487 Human
Entrez Gene: 11937 Mouse
Entrez Gene: 100037716 Rabbit
Omim: 108730 Human
SwissProt: O14983 Human
SwissProt: Q8R429 Mouse
SwissProt: P04191 Rabbit
Unigene: 657344 Human
Unigene: 35134 Mouse
Unigene: 217139 Rat
Product Picture Paraformaldehyde-fixed, paraffin embedded (rat heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AT2A1) Polyclonal Antibody, Unconjugated (SL7539R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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