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Product Name FAIM3 Chinese Name FAS凋亡抑制分子3抗体 Alias Faim3; FAIM3_HUMAN; Fas apoptotic inhibitory molecule 3; Fc mu receptor; FCMR; IgM Fc receptor; Immunoglobulin mu Fc receptor; Regulator of Fas induced apoptosis; Regulator of Fas-induced apoptosis Toso; TOSO. Research Area Cell biology Signal transduction Apoptosis Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 41kDa Cellular localization The cell membrane Secretory protein Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human FAIM3: 61-160/390 <Extracellular> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this May play a role in the immune system processes. Protects cells from FAS-, TNF alpha- and FADD-induced apoptosis without increasing expression of the inhibitors of apoptosis BCL2 and BCLXL. Seems to activate an inhibitory pathway that prevents CASP8 activation following FAS stimulation, rather than blocking apoptotic signals downstream. May inhibit FAS-induced apoptosis by preventing CASP8 processing through CFLAR up-regulation.
Function:
May play a role in the immune system processes. Protects cells from FAS-, TNF alpha- and FADD-induced apoptosis without increasing expression of the inhibitors of apoptosis BCL2 and BCLXL. Seems to activate an inhibitory pathway that prevents CASP8 activation following FAS stimulation, rather than blocking apoptotic signals downstream. May inhibit FAS-induced apoptosis by preventing CASP8 processing through CFLAR up-regulation.
Subcellular Location:
Isoform 1: Membrane; Single-pass membrane protein.
Isoform 3: Secreted. Note=Detected in the serum.
Tissue Specificity:
Expressed in lymph nodes, peripheral blood leukocytes, lung, thymus and kidneys. Very weak expression detected in spleen, liver, heart, and salivary gland. Expressed in lymphoid cell lines such as Jurkat, CEM-T4, MOLT-4, HB11;19 and Reh. No expression detected in nonhematopoietic cell lines including Hep-G2, HEK293 and HeLa. Detected at high levels in chronic lymphocytic leukemia cells.
Similarity:
Contains 1 Ig-like (immunoglobulin-like) domain.
SWISS:
O60667
Gene ID:
9214
Database links:Entrez Gene: 9214 Human
Omim: 606015 Human
SwissProt: O60667 Human
Unigene: 58831 Human
Unigene: 728324 Human
Product Picture Sample:
Lymph node (Mouse) Lysate at 40 ug
Primary: Anti- FAIM3 (SL7527R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 41 kD
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FAIM3) Polyclonal Antibody, Unconjugated (SL7527R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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